Hyp (Hydroxyproline) ELISA Kit - 96 wells plate

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432,00 € 432.0 EUR 432,00 € Exclusief btw

432,00 € Exclusief btw

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    Product name:Hyp (Hydroxyproline) ELISA Kit
    Alternative Names:(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)
    Assay Type:Competitive Inhibition
    Sensitivity:26.3 ng/mL
    Standard:5000 ng/mL
    Range:78.13-5000 ng/mL
    Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay Length:2h
    Research Area:Metabolic pathway;Nutrition metabolism;
    Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hydroxyproline(Hyp) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxyproline(Hyp). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxyproline(Hyp) in the samples is then determined by comparing the OD of the samples to the standard curve.