[AFG-E9107] AffiELISA® Human NTXI (Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit - 96-wells plate

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    Product name:Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit
    Reactivity:Human
    Alternative Names:NTX-I; NTX1
    Assay Type:Competitive Inhibition
    Sensitivity:0.94 ng/mL
    Standard:200 ng/mL
    Detection Range:3.13-200 ng/mL
    Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay Length:2h
    Research Area:Metabolic pathway; Bone metabolism;
    Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTXI in the samples is then determined by comparing the OD of the samples to the standard curve.


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