Disease: Carcinoma
Origin: This breast tumor line was established in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices.
Species: Homo sapiens, human
Tissue: Breast, mammary gland
Properties: Epithelial, adherent
Complete Medium: Formulated EMEM (C0005-01)[compare formulation before culturing if not using our medium C0005-01, please see remarks below] + 10% FBS
Subculture Procedure: 1:2 -1:4 using trypsin/EDTA; culture at 5% CO2, 37°C
Medium Renewal: Two to three times weekly.
Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO