MIA-PACA-2 cell line - 1 vial

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3.790,00 € 3790.0 EUR 3.790,00 € Exclusief btw

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    Verzending: 2 tot 3 weken

    The MIA PaCa-2 cell line was established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male in 1975 by A. Yunis, et al. These cells are described to express human colony stimulating factor, subclass I (CSF-I) and plasminogen activator. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Sensitive to L-Asparaginase.

    Species: Homo sapiens (human)

    Tissue of Origin: Pancreas

    Recommended Medium: DMEM + 10% h.i.FBS

    Culture Properties: monolayer with floating cells

    Morphology: Epithelial-like

    STR DNA Profile:

    Amelogenin: X

    CSF1PO: 10

    D13S317: 12,13

    D16S539: 10,13

    D5S818: 12,13

    D7S820: 12,13

    THO1: 9,10

    TPOX: 9

    vWA: 15

    Karyotype: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

    Applications: The MIA PaCa-2 cell line can be used as tumourigenicity and enzymatic studies.

    Disease: Carcinoma

    Quality Control: Tests for mycoplasma, bacteria and fungi were negative

    Storage and Shipping: Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen