Concentrating Solution, 50ml
Product details
DESCRIPTION: Sample concentration solution for Chondrex, Inc.’s Sirius Red Total Collagen Detection Kit (Cat # 9062)
QUANTITY: 50 ml
FORM: Polyethylene glycol solution
USAGE: Collagen levels in culture media are generally lower than the standard range of the Sirius Red Total Collagen Detection Kit (Cat # 9062). This concentrating solution (Cat # 90626) and the following protocol can be used for concentrating culture media samples.
1. Take 1 ml culture medium.
2. Add 250 µl of Concentrating Solution
3. Vortex and incubate at 4°C for 16-24 hours.
4. Centrifuge at 10,000 rpm for 3 minutes. 5. Discard supernatant.
6. Add 100 µl of 0.05M acetic acid to dissolve the pellet. Use this solution as your sample.
7. The calculated collagen concentration should be multiplied by a 0.1 dilution factor
STORAGE: -20°C
STABILITY: 2 years
NOTES: N/A
REFERENCES: N/A