Immunization Grade Bovine Type II Collagen, Solution (2 mg/ml x 5 ml)

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    Immunization Grade Bovine Type II Collagen, Solution (2 mg/ml x 5 ml)

    Product details

    DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4)

    APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals. 

    QUANTITY: 5 ml

    FORM: 2 mg/ml solution in 0.05M acetic acid 

    SOURCE: Bovine articular cartilage

    MOLECULAR WEIGHT: 300 kDa

    PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans

    STORAGE: -20°C in the dark

    STABILITY: 2 years

    NOTES: The maximum concentration of type II collagen in acidic buffer is 4 mg/ml. In order to neutralize this product, add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (NaCl final concentration: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.

    NOTE: Although type II collagen does not form gel at low concentrations, Chondrex, Inc. recommends keeping the collagen solution on ice. 

    REFERENCES:

    R. Trelstad, et al. Biochemistry 9: 4993-4998 (1970)

    E. Miller, Biochemistry 10: 1652-1659 (1971) 

    D. Trentham, et al. J. Exp. Med. 146: 857-868 (1977)

    P. Wooley, et al. J. Immunology 135: 2443-2451 (1985)