Human brucella antibody (IgM) Qualitative ELISA kit - 96 wells plate
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with avidin and biotin-conjugated antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human brucella antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay has high sensitivity and excellent specificity for detection of human brucella antibody (IgM). No significant cross-reactivity or interference between human brucella antibody (IgM) and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human brucella antibody (IgM) and all the
analogues, therefore, cross reaction may still exist.