Methylamp MS-qPCR Fast Kit - 200 Reactions
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640,00 € 640.0 EUR 640,00 € Exclusief btw

640,00 € Exclusief btw

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    Methylamp MS-qPCR Fast Kit - 200 Reactions

    The Methylamp™ MS-qPCR Fast Kit is a complete set of essential components, which enables the experimenter to perform fast, specific, sensitive and reproducible methylation-specific quantitative PCR. The Methylamp™ MS-qPCR Fast Kit is very suitable for quantitative methylation-specific PCR in a fast format using very minute amounts of DNA. The novel hot start DNA polymerase contained in this kit can specifically reduce the overall time required for MS-qPCR from approximately 2.5 hours to less than 70 minutes. The kit also facilitates sensitivity and specificity of MS-qPCR by significantly increasing primer-bisulfite DNA template annealing, while simultaneously reducing non-specific annealing. This results in considerable time savings and a more efficient MS- qPCR. The kit has the following advantages:

    • An extremely fast procedure that can be finished within 70 minutes.
    • Abundant yields due to high amplification efficiency.
    • Highly accurate and specific in MSP, with reduced false- positive results.
    • Convenient master mix format to allow easy reaction setup.
    • Simple, reliable, and consistent assay conditions.
    • Can be used with any block-based real-time PCR instrument.

    Principle & Procedure
    The Methylamp™ MS-qPCR Fast Kit provides a master mix format, which contains a hot start DNA polymerase, dNTPs, an MS-PCR enhancer, an optimized buffer, and an intercalating green dye. This master mix allows for a convenient and easy reaction setup. The unique hot start DNA polymerase is provided in an inactive state at ambient temperature and is reactivated by several minute incubations at 95°C, which can easily be integrated into existing thermal cycling steps. The hot start DNA polymerase in combination with the optimized buffer ensures the MS-qPCR specificity and sensitivity. The green dye allows for DNA detection and analysis without using a sequence-specific probe.