ASSAY PROCEDURE SUMMARY:
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at
37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.
6. Calculate.