CytoFix™ Red Lysosomal Stain

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524,00 € 524.0 EUR 524,00 €

524,00 €

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    Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste and cellular debris through a process known as autophagy. AAT Bioquest offers CytoFix™ Red lysosomal stain for selectively staining lysosomes. CytoFix™ Red lysosomal stain is well retained in lysosomes even after fixation. The dye permeates intact live cells and gets trapped in lysosomes. The fluorescence in lysosomes generated by this dye is well retained at least for 1 week, making it an excellent lysosomal tracking dye. The key features of this stain are its high staining efficiency, long retention after fixation with minimal hands on time. CytoFix™ Red lysosomal stain can be used with GFP expressed cells or with other organelles stains for multicolor analysis. It can be used for both suspension and adherent cells and readily adapted for a wide variety of fluorescence platforms.

    Platform


    Fluorescence microscope

    ExcitationCy3/TRITC filter set
    EmissionCy3/TRITC filter set
    Recommended plateBlack wall/clear bottom
    Instrument specification(s)Cy3/TRITC filter set

    Calculators


    Common stock solution preparation

    Table 1. Volume of DMSO needed to reconstitute specific mass of CytoFix™ Red Lysosomal Stain to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

    0.1 mg0.5 mg1 mg5 mg10 mg
    1 mM115.774 µL578.871 µL1.158 mL5.789 mL11.577 mL
    5 mM23.155 µL115.774 µL231.548 µL1.158 mL2.315 mL
    10 mM11.577 µL57.887 µL115.774 µL578.871 µL1.158 mL

    Molarity calculator

    Enter any two values (mass, volume, concentration) to calculate the third.

    Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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    Spectrum


    Open in Advanced Spectrum Viewer

    Spectral properties

    Images


    Figure 1. The fluorescence images of HeLa cells stained with CytoFix™ LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.
     

    Documents


    Safety data sheet
    Product protocol
    Certificate of analysis

    Application notes


    A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
    Abbreviation of Common Chemical Compounds Related to Peptides
    Annexin V
    Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
    Calcein