Highly Sensitive 8-OHdG Check ELISA kit

Alias: KOG- HS10E

Price contact : support@gentaur.com

About 8-hydroxy-2'-deoxyguanosine (8-OHdG):

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals.

Specifications

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Measuring range: 0.125 to 10 ng/mL
Format: 96 wells (18 samples in triple assays)
Applications: Urine, serum, tissue and cultured cells.
Storage: Store at 4 - 10°C (don't freeze).
Expiry: 9 months after the day of manufacturing.
Required but not provided: Micropipet and chip (100 µL, 1000 µL).
Measuring pipet (10 mL, 20 mL)/ measuring cylinders.
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
Microplate reader (filter; 450 nm).

Content of this kit

8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type) 1 plate
Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial
Primary Antibody Solution   1 vial (6mL)
Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial
Secondary Antibody Solution:   1 vial (12mL)
Chromatic Solution: 3,3',5,5'-tetramethylbenzidine 1 vial (0.25mL)
Diluting Solution: H2O2 containing buffer. 1 vial (12mL)
Washing Solution(5x):   2 vials (26mLx2)
Reaction Terminating Solution: 1M Phosphoric acid. 1 vial (12mL)
Standard 8-OHdG Solution: Purified 8-OHdG (0.125, 0.25, 0.5, 1, 4, 10 ng/mL) 1 vial each
Plate Seal:   2 sheets