P24 HIV Antigen ELISA

 

Alias: 0801111

Price contact : support@gentaur.com

At the forefront of HIV Research: Basic Virology and Immunology, Detection, Diagnosis, Treatment and Monitoring

The Alliance HIV-1 p24 Antigen ELISA®* is for the detection of HIV-1 and HIV-0 subtypes in human serum, plasma, cell culture supernates and dry blood spots (DBS). This kit provides reagents for immune complex dissociation (ICD) of antigen/antibody complexes in serum, plasma and dry blood spot samples and proprietary antibodies increasing the sensitivity of the assay. The ELAST™ p24 ELISA Amplification System also improves the sensitivity of Alliance HIV-1 p24 ELISA kits by approximately 25-fold while extending the dynamic range of the assay.

Feature and Benefits

  • Increased Sensitivity for low viral load
    • 100 fg to 6,250,000 fg detection curve (12.5 to 200 pg/mL), translates into 12-fold increase in sensitivity
  • Sample matrix: Serum, Plasma, Cell Culture Supernate and Dry Blood Spots (DBS)
  • Pure p24 positive control (200 ng/mL) that allows curve flexibility
    • Standard Curve: from 12.5 pg to 100 pg
    • ICD Curve: from 25 pg to 400 pg
    • Upper Stretched curve: from 12.5 pg or 25 pg to 4000 pg
    • Lower Stretched curve: from 3.5 pg to 100 pg, 400 pg or 4000 pg
  • Low sample volume requirement (50 uL)
  • Complete Kit, ready to use

This kit is intended for research purposes only and is not approved for diagnostic use.

 

INTENDED USE


The RETRO-TEK HIV-1 p24 Antigen ELISA is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease, or for screening and may not be used as a confirmatory test in diagnostic situations.
The RETRO-TEK HIV-1 p24 Antigen ELISA is an enzyme linked immunoassay used to detect Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen in research specimens, including cell culture media, as well as human sera and plasma. It can also be used to monitor the purification and biochemical behavior of HIV-1. Furthermore, the assay may augment or supplant reverse transcriptase measurements traditionally employed to detect the presence of HIV-1. Such enzymatic measurements are not HIV-1 specific.
The RETRO-TEK HIV-1 ICx/CRx Kit (ZMC Catalog #: 0801096) for immune complex dissociation and confirmation of p24 reactivity enhances the detection of p24 antigen in serum or plasma. Many specimens contain HIV-1 viral loads greater than the range of the standard curve of this assay.

The RETRO-TEK HIV-1 p24 Extended Range ELISA (ZMC catalog # 0801137) will extend the standard curve of this assay to 4 ng/ml

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PRINCIPLE OF THE TEST


Microwells are coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1. Viral antigen in the specimen is specifically captured onto the immobilized antibody during specimen incubation. The captured antigen is then reacted with a hightitered human anti-HIV-1 antibody conjugated with biotin. Following a subsequent incubation with Streptavidin-Peroxidase, color develops as the bound enzyme reacts with the substrate. Resultant optical density is proportional to the amount of HIV-1 p24 antigen present in the specimen.


REAGENTS
Materials Supplied:
• HIV-1 p24 Antibody Coated Microplate for 96 determinations,1 plate: 12x8 well strips.
• HIV-1 p24 Detector Antibody, 1 vial, lyophilized: Contains biotin-labeled human antibody to HIV-1, human source material non-reactive for antibodies to HIV-1 and non-reactive for HIV-1 p24 antigen, milk, Triton X-100®, Tween 20®, and PBS.
• Detector Antibody Diluent: 25 ml
• HIV-1 p24 Antigen Standard, 0.5 ml: Contains detergent-disrupted, heatinactivated viral antigen, goat serum, Triton X-100®, and sodium azide.
• Lysing Buffer, 5 ml: Triton X-100® in PBS and 2-chloroacetamide.
• Streptavidin-Peroxidase, 0.3 ml: Contains streptavidin conjugated to horseradish peroxidase, PBS, goat serum, and 2-chloroacetamide. Assay Diluent, 100 ml: Contains goat serum, PBS, Triton X-100®, and 2-chloroacetamide.
• 10X Plate Wash Buffer, 125 ml: Contains PBS, Tween 20®, and 2-chloroacetamide.
• Substrate, 0.3 ml: Contains tetramethylbenzidine (TMB), and dimethyl sulfoxide (DMSO).
• Substrate Buffer, 50 ml: Contains citrate/acetate buffer, hydrogen peroxide, and 2- chloroacetamide.
• Stop Solution, 12 ml: Proprietary formulation.
• Plate Sealers: 10
• Resealable Plastic Bag: 1
® Triton X-100 is a registered trademark of Union Carbide Chemicals and Plastics Co., Inc. Tween
20 is a registered trademark of Imperial Chemical Industries.